pAAV-RC2 Vector - 10 µg
Product details
Background
Adeno-associated viruses (AAVs) are derived from defective parvoviruses, which depend on essential
helper functions provided by other viruses, such as adenovirus and herpes virus, for efficient viral
replication and propagation. AAV has no etiologic association with any known diseases and has been
successfully used to establish efficient and long-term gene expression in vivo in a variety of tissues
without significant cellular immune responses or toxicity.
AAV has a single-stranded DNA genome which consists of approximately 4.7 kb. All characterized
AAV serotypesshare three key features, including two copies of AAV terminal repeats (ITRs), one rep
region and one cap region. The ITRs are capable of forming T-shape secondary structure and are the
only cis elements that are required for AAV replication, packaging, integration, and rescue. The rep
region encodes four overlapping proteins designated as Rep78, Rep68, Rep52, and Rep40, according to
the apparent molecular mass of the protein. In addition to their well-defined roles in AAV replication,
Rep proteins also regulate AAV packaging and site-specific integration. The cap region encodes three
structural proteins, VP1, VP2, and VP3. These three proteins share the same reading frame (see Figure
1).