A sandwich enzyme immunoassay for in vitro quantitative measurement of DPP8 in human serum, plasma, tissue homogenates and other biological fluids.
This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Dipeptidyl peptidase 8 (DPP8). Add
osteoclast differentiation factor (DPP8) to the wells, which are pre-coated with osteoclast differentiation factor (DPP8) monoclonal antibody and then incubate. After that, add anti DPP8 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Dipeptidyl peptidase 8 (DPP8) are positively correlated.
ASSAY PROCEDURE SUMMARY:
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at
37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10minutes.
6. Calculate.