Mouse Hantavirus IgG (HV-IgG) ELISA Kit (Qualitative) - 96 wells plate

Purpose:

Our Mouse Hantavirus IgG (HV-IgG) ELISA Kit is to for the qualitative determination of HV-IgG in Mouse serum, plasma, culture media or any biological fluid.

Principle:

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to HV-IgG, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for HV-IgG is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain HV-IgG and HRP conjugated HV antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HV-IgG is determined by comparing with the CUTOFF value.