Description: Staphylococcus aureus alpha hemolysin
(Hla), also known as alpha toxin (α-toxin), is expressed
in E. coli as a recombinant protein without TAG. The
theoretical molecular weight of the protein is
approximately 33 kDa.
Supplied: 100 µg is supplied in phosphate buffered
saline and 20% glycerol, at a concentration of
1.489 mg/mL.
Storage: -80°C
Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of Hla protein using IBT’s mouse anti-Alpha Toxin (6C12) monoclonal antibody (cat# 0210-005) in Western blotting.
Cytotoxicity assay: The protein is active in functional
lysis assays with rabbit red blood cells. The protein can be also used in serology assay as coating antigen to detect antibodies against Hla.