Human dipeptidyl peptidase 8 (DPP8) ELISA Kit - 96-wells plate

A sandwich enzyme immunoassay for in vitro quantitative measurement of DPP8 in human serum, plasma, tissue homogenates and other biological fluids.

This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Dipeptidyl peptidase 8 (DPP8). Add

osteoclast differentiation factor (DPP8) to the wells, which are pre-coated with osteoclast differentiation factor (DPP8) monoclonal antibody and then incubate. After that, add anti DPP8 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Dipeptidyl peptidase 8 (DPP8) are positively correlated.

ASSAY PROCEDURE SUMMARY:

1. Prepare all reagents, samples and standards;

2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.

3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at

37 ℃; for color development.

4. Add stop solution

5. Read the OD value within 10minutes.

6. Calculate.