Yeast Total RNA - 0.025mg
Yeast Total RNA is extracted from freshly harvested Saccharomyces cerevisiae yeast using classical guanidine isothiocyanate–phenol: chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase-1 to remove residual DNA, precisely quantified by NanoDrop, and stored at -80oC.
The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.
Total RNA sample is provided in RNase-free water at a concentration of 1mg/ml and shipped on dry ice.