EvaGreen® Dye, 20X in Water - Trial size (1 mL)

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185,00 € 185.0 EUR 185,00 € hors TVA

185,00 € hors TVA

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    Conditions générales
    Garantie satisfait ou remboursé de 30 jours
    Expédition : 2-3 jours ouvrables

    EvaGreen® Dye is a patented green fluorescent nucleic acid dye that is essentially non-fluorescent by itself, but becomes highly fluorescent upon binding to dsDNA.  It is ideal for a wide variety of applications including qPCR and DNA melt curve analysis, HRM®, LAMP, digital PCR, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), capillary gel electrophoresis, and more. The dye is provided at 20X (25 uM) in water.

    • Brighter and less inhibitory than SYBR® Green
    • Environmentally safe, non-mutagenic, and non-cytotoxic
    • Unsurpassed thermal stability, chemical stability, and photostability
    • Excellent compatibility with multiplex PCR
    • Directly visualize amplification products in a gel
    • Compatible with common qPCR instruments and enzyme systems

    The Unique Properties of EvaGreen® Dye

    EvaGreen® Dye was designed with a novel “release-on-demand” mechanism. In the absence of DNA, the dye assumes a conformation that is inactive. When DNA is available, the dye shifts to an active conformation that binds DNA to emit fluorescence. This property of the dye provides a unique mechanism to continuously supply the active form of the dye from an inactive reserve, as more DNA is produced during the PCR process. As a result, EvaGreen® Dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for digital PCR, real-time qPCR, HRM®, and multiplex qPCR by DNA melt curve analysis. This technology also makes it possible to significantly shorten the chain extension time, enabling the use of fast PCR protocols. 


    Detection method/readout

    PCR/qPCR, HRM®


    500/530 nm (with DNA)


    20X (25 uM)

    Storage Conditions

    Store at -10 to -35 °C