Mouse SAA ELISA Kit - 96-wells plate

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    he principle of the double antibody sandwich Mouse SAA (serum amyloid a) ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-Mouse SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. 


    Reactivity: Murine/Mouse
    Specificity: Target Serum Amyloid A
    Size: 96 wells
    Detection Range: 31.25 ng/ml - 2000 ng/ml
    Sensitivity: 11.015 ng/ml
    Assay Time: 100 min.
    Sample Types: Plasma, Serum
    Storage: 2-8C


    Citations:

    Ménoret A et al. Trace Levels of Staphylococcal Enterotoxin Bioactivity Are Concealed in a Mucosal Niche during Pulmonary Inflammation. (2015) PLoS ONE 10(10): e0141548. doi:10.1371/journal.pone.0141548.

    Brancaleone et al. A vasculo-protective circuit centered on lipoxin A4 and aspirin-triggered 15-epi-lipoxin A4 operative in murine microcirculation Blood: 122 (4) Pages: 608 - 617 DOI: http://dx.doi.org/10.1182/blood-2013-04-496661