Recombinant GFP, C-terminal 6xHis-tag - 100 μg/vial at 1.0 mg/mL in 1X PBS
Product details
Background
Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the
jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its subsequent expression in
heterologous systems have established recombinant GFP (rGFP) as a valuable reporter molecule for in
vivo visualization of gene expression events in a wide variety of cell types and organisms. GFP can fuse
with proteins of interest without interfering significantly with their assembly and function. Based on the
structure of the GFP molecule, many GFP variants have been created with much improved fluorescence
emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and
flow cytometry. Since rGFP requires no additional substrates or cofactors, rGFP fluorescence can be
easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.