Maltose Binding Protein (MBP-Tag) Monoclonal antibody [Clone: 1E52C2] - 100 ug

Alternative Names: Maltose Binding Protein, MBP, MBP Tag

Raised in: Mouse

Immunogen: MBP-Tag fusion protein

Conjugate: Non-conjugated

Clonality: Monoclonal

Isotype: IgG2a

Clone No.: 1E52C2

Purity: >95%,Protein G purified

Concentration: Batch dependent

Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Tested Applications: ELISA, WB

Recommended Dilution (WB): 1:500-1:5000

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze-thaw cycles.

Background:

The MBP monoclonal antibody is produced by immunizing mice with the MBP-Tag fusion protein and then fusing B cells isolated from the immunized mice with myeloma cells to create hybridomas. After screening, the best hybridoma cell line is cultured in the mouse abdominal cavity. The MBP monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography to ensure a purity of over 95%. This unconjugated IgG2a antibody is ideal for detecting MBP-tagged proteins in ELISA and WB applications.

MBP is a cytoplasmic protein that binds specifically to maltose and other sugar molecules. It is commonly used as a fusion partner for recombinant protein expression and purification, allowing for efficient purification of the fused protein using immobilized metal ion affinity chromatography (IMAC) or amylose resin. MBP has also been shown to have chaperone activity and can enhance the solubility and stability of fusion proteins. In addition, MBP has been used as a tool in studies of protein-protein interactions and protein folding.

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