Mouse Anti-OVA IgG Antibody Assay Kit

Mouse Anti-OVA IgG Antibody Assay Kit

Product details

DESCRIPTION: ELISA kit to quantify mouse anti-ovalbumin antibodies

FORMAT: 96-well ELISA Plate with removeable strips 

ASSAY TYPE: Indirect ELISA

ASSAY TIME: 4.5 hours

STANDARD RANGE:

3011 (IgG) : 12.5 - 0.2 ng/ml 

3013 (IgG1) : 12.5 - 0.2 ng/ml 

3015 (IgG2a) : 50 - 0.8 ng/ml

3016 (IgG2b) : 50 - 0.8 ng/ml

3017 (IgM) : 100 - 1.6 ng/ml

3018 (IgA) : 12.5 - 0.2 ng/ml

3029 (IgG2c) : 50 - 0.8 ng/ml

NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate

SAMPLE TYPES: Serum & Plasma 

RECOMMENDED SAMPLE DILUTIONS: 1:100 (at least) 

CHROMOGEN: TMB (read at 450 nm)  

STORAGE: -20°C for 12 months

VALIDATION DATA: 

3011: Intra-Assay (2.6-9.5%)/Inter-Assay (0.4-3.7%)/Spiking Test (93-107%) 

3013: Intra-Assay (4.7-9.1%)/Inter-Assay (3.6-9.5 %)/Spiking Test (96-114%)

3015: Intra-Assay (5.9-8.9%)/Inter-Assay (6.3-9.4%)/Spiking Test (98-104%

3016: Intra-Assay (3.9-9.9%)/Inter-Assay (6.3-9.4%)/Spiking Test (94-106%)

3017: Intra-Assay (2.9-4.6%)/Inter-Assay (6.6-9.1%)/Spiking Test (93-116%)

3018: Intra-Assay (0.2-7.8%)/Inter-Assay (1.2-7.9%)/Spiking Test (93-98%) 

3029: Intra-Assay (5.5-9.6%)/Inter-Assay (2.9-7%)/Spiking Test (100-109%)

NOTES: N/A