The MIA PaCa-2 cell line was established from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male in 1975 by A. Yunis, et al. These cells are described to express human colony stimulating factor, subclass I (CSF-I) and plasminogen activator. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. Sensitive to L-Asparaginase.
Species: Homo sapiens (human)
Tissue of Origin: Pancreas
Recommended Medium: DMEM + 10% h.i.FBS
Culture Properties: monolayer with floating cells
Morphology: Epithelial-like
STR DNA Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15
Karyotype: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.
Applications: The MIA PaCa-2 cell line can be used as tumourigenicity and enzymatic studies.
Disease: Carcinoma
Quality Control: Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping: Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen