Nanaomycin A (DNMT3B inhibitor) - 25 mg

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1 360,00 €

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    Conditions générales
    Garantie satisfait ou remboursé de 30 jours
    Expédition : 2-3 jours ouvrables

    Chemical Properties

    Physical AppearanceA solid
    StorageStore at -20°C
    M.Wt302.28
    Cas No.52934-83-5
    FormulaC16H14O6
    SynonymsNanafrocin, Nanafrocine, Nanafrocinum, Rosanomycin A
    Solubilityinsoluble in H2O; ≥15.1 mg/mL in DMSO; ≥31.07 mg/mL in EtOH with ultrasonic
    Chemical Name2-[(1S,3S)-9-hydroxy-1-methyl-5,10-dioxo-3,4-dihydro-1H-benzo[g]isochromen-3-yl]acetic acid
    SDFDownload SDF
    Canonical SMILESOC1=CC=CC(C(C2=C3[C@H](C)O[C@H](CC(O)=O)C2)=O)=C1C3=O
    Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
    General tipsWe do not recommend long-term storage for the solution, please use it up soon.


    Protocol

    Cell experiment[1]:

    Cell lines

    A549, HL60, HeLa and HCT116 cells 

    Preparation method

    The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

    Reaction Conditions

    Ranging from 10 nM to 10 μM for 72 h

    Applications

    Nanaomycin A, initially identified by a virtual screening for inhibitors against DNMT1, as a compound inducing antiproliferative effects in three different tumor cell lines originating from different tissues. Nanaomycin A treatment reduced the global methylation levels in all three cell lines and reactivated transcription of the RASSF1A tumor suppressor gene. In biochemical assays, nanaomycin A revealed selectivity toward DNMT3B.

    Animal experiment [2]:

    Animal models

    Guinea pigs

    Applications

    The therapeutic effect of nanaomycin A and siccanin against experimental cutaneous Trichophyton mentagrophytes infection in guinea pigs was investigated. Topically applied formulation of nanaomycin A was very effective in improving the condition of lesions and in preventing fungal growth in the infected tissues. Nanaomycin A and siccanin were comparable in activity in experiments.

    Other notes

    Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

    References:

    1. Kuck D1, Caulfield T, Lyko F et al. Nanaomycin A selectively inhibits DNMT3B and reactivates silenced tumor suppressor genes in human cancer cells. Mol Cancer Ther. 2010 Nov;9(11):3015-23.

    2. Kitaura K, Araki Y, Marumo H. The therapeutic effect of nanaomycin A against experimental Trichophyton mentagrophytes infection in guinea pigs. Kitaura K, Araki Y, Marumo H.

    Biological Activity

    DescriptionNanaomycin A is a selective inhibitor of DNMT3B.
    TargetsDNMT3B     
    IC50500 nM