bEnd.3 (BEND3) cells (endothelial cells isolated from brain tissue derived from a mouse with endothelioma) - 1 vial

https://www.gentaur.be/web/image/product.template/7459/image_1920?unique=7cf6726
(0 avis)

1 027,00 € 1027.0 EUR 1 027,00 € hors TVA

1 027,00 € hors TVA

Not Available For Sale

    Cette combinaison n'existe pas.

    Conditions générales
    Garantie satisfait ou remboursé de 30 jours
    Expédition : 2-3 jours ouvrables

    Disease:

    Endothelioma

    Origin:

    The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.

    Species:

    Mus musculus, mouse

    Tissue:

    Brain, cerebral cortex

    Morphology:

    Endothelial, endothelialpolyoma middle T antigen transformed

    Properties:

    Adherent

    Strain:

    BALB/c, 6 weeks

    Medium:

    DMEM (0683-C0003-01) + 10% FBS

    Subculture:

    Subculture sub-confluent cultures (70-80%) at 1:6 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

    Freezing Medium:

    Complete growth culture medium supplemented with 7.5% (v/v) DMSO

    Biosafety Level:

    I

    Sterility:

    Bacteria: Negative

    Yeast: Negative

    Mycoplasma: Negative

    Pathogens:

    HIV: Negative

    Hepatitis B: Negative

    Size:

    1 vial of ~1x10^6 cells (Lot dependent)

    References for bEnd.3 cells:

    1. Williams RL, Courtneidge SA, Wagner EF. Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell. 1988 Jan 15;52(1):121-131. PMID: 3345558