Reactivity: Human TGF-beta
Range: 4 pg/ml -300 pg/ml
Sensitivity: 0.8 pg/ml
Principle:
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa strip plate provided in this kit has been pre-coated with an antibody specific to TGF-β. Standards or samples are added to the appropriate Microelisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TGF-β is added to each Microelisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TGF-β and HRP conjugated TGF-β antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TGF-β. You can calculate the concentration of TGF-βin the samples by comparing the OD of the samples to the standard curve.