pUC57-Kanamycin Information
Promoter: Lac/lac
Replicon: ColE1 ori
Plasmid classification: Escherichia coli vector; cloned plasmid
Plasmid size: 2579bp
Plasmid label: LacZ
Prokaryotic resistance: kanamycin Kan
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
5'sequencing primers: M13R:CAGGAAACAGCTATGACC
3'sequencing primers: M13F:TGTAAAACGACGGCCAGT
Note: selection of blue leukoplakia
Sequence:
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg
481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
1081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
1201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
1321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
1501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaagccca
1621 atctgaataa tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
1681 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
1741 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
1801 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
1861 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
1921 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
1981 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
2041 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
2101 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
2161 ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
2221 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
2281 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
2341 atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
2401 atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttgaatatg
2461 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
2521 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgca
