Panspecies Carnitine (VB20, Vitamin B20) ELISA Kit - 48 wells plate

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    Product name: CNT (Carnitine) ELISA Kit

    Reactivity: General

    Alternative Names: VB20; Vitamin B20

    Assay Type: Competitive Inhibition

    Sensitivity: 4.84 ng/mL

    Standard: 1000 ng/mL

    Range: 15.63-1000 ng/mL

    Sample Type: serum, plasma and other biological fluids

    Assay Length: 2h

    Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CNT in the samples is then determined by comparing the OD of the samples to the standard curve.