Nori® Salmon Leptin ELISA Kit - 96-wells plate
This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme-linked immunoassay technique and uses
chemistry to improve the performance of
the assays. An antibody specific for leptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any leptin present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for leptin is added to the wells. Following wash to remove any unbound antibody, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of leptin bound in the initial step. The color development is stopped and the intensity of the color is measured.